Category: 2.o™ HPLC Columns FAQ
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Aqueous Normal Phase ANP ballistic gradients compared to same in HILIC - Tips & Suggestions
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Are there HPLC columns that could be used to separate both polar and nonpolar compounds in a single run - FAQ
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Determining Efficiency of HPLC methods comparing 4um v 2.o HPLC columns - Tips & Suggestions
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Do Cogent TYPE-C Silica columns have a partial positive charge and if so is retention for basic compounds reduced because of this - Tech Information
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Eliminate broad split peaks in Aqueous Normal Phase ANP methods and a methanol diluent- Tips & Suggestions
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For the separation of polar intermediates in reversed phase HPLC which C18 column do you recommend - FAQ
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High molecular weight compounds that won't retain on Cogent Diamond Hydride column try Cogent Bidentate C8 300 - Tips & Suggestions
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How can I restore my Diamond Hydride HPLC column after I ran out of mobile phase and air was accidently introduced into it - FAQ
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How do I avoid distorted peak shapes when using a basic mobile phase pH for the analysis of a lipophilic amine - FAQ
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How do retention and efficiency of asymmetric dimethylarginine ADMA compare using 4um vs 2.o Diamond Hydride columns - AppNote
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How often do I need to do the conditioning procedure for a new Diamond Hydride column - FAQ
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How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
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How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
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I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column - Tips & Suggestions
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I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds - FAQ
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Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns - FAQ
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Lower retention is observed when functional group in a compound is ionized - Tips & Suggestions
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Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method - Tips & Suggestions
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Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts - Tips & Suggestions
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Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
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Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP - FAQ
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Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
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Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ
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The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase - Tips & Suggestions
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Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
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Using two HPLC columns together in a tandem separation for polar and non polar retention - Tips & Suggestions
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What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies - FAQ
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What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
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What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments - FAQ
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What pressure should I expect with a Diamond Hydride 2.o HPLC column at flow rates from 0.2 to 2.0mL / min - FAQ
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What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column - FAQ
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Which column do you suggest for Cocaine & metabolite Ecognin separation - Tips & Suggestions
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Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents - FAQ
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